SIRT6 Inhibitor Screening Kit (Fluorometric)
货号：
K323-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K323-100 |
| 描述 | SIRT6 or Sirtuin 6 proteins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. SIRT6 is a nuclear sirtuin that has been associated with aging, cellular protection, sugar metabolism and certain types of cancer. Broad therapeutic applications are foreseen for SIRT6 inhibitors, including uses in diabetes, immune-mediated disorders, and cancer. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In BioVision’s Sirtuin 6 inhibitor screening Kit, Sirtuin 6 deacetylates the substrate, followed by cleavage of the deacetylated substrate to release the fluorescent group, which is detected fluorometrically at Ex/Em = 395/541 nm. In the presence of SIRT inhibitor, deacetylation is impeded, preventing cleavage of the substrate and release of the fluorescent group. This kit provides a rapid, simple, sensitive, and reliable test, which is suitable for high-throughput screening of SIRT6 inhibitors. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 395/541 nm)• Applications - Screening/characterizing/studying SIRT6 inhibitors |
| 产品特色 | • Simple and reliable test to screen SIRT6 inhibitors• High-throughput suitable• Includes Inhibitor Control (Nicotinamide) |
| 试剂盒组分 | • SIRT6 Assay Buffer• Substrate• Cofactor • Developer • SIRT6 Enzyme • Enzyme Reconstitution Buffer• Inhibitor (Nicotinamide, 4 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
