PicoProbe™Acetyl-CoA Fluorometric Assay Kit
货号：
K317-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K317-100 |
| 描述 | Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates. BioVision has developed a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence (Ex=535/Em=587 nm). The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The assay can detect 10 to 1000 |
| 产品特色 | • Very simple procedure • Convenient • BioVision has developed a high sensitivity assay for Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence. |
| 试剂盒组分 | • Acetyl CoA Assay Buffer • PicoProbe • Conversion Enzyme • Acetyl CoA Enzyme Mix • Acetyl CoA Substrate Mix • CoA Quencher • Quench Remover • Acetyl CoA Standard (10 µmol) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
