| 货号 | K224-100 |
| 描述 | α-L-Fucosidase (EC 3.2.1.51) (FUCA1) is a hydrolase that is able to cleave α-L-fucosyl moieties from glycoconjugates and oligosaccharides. Fucosidase plays pivotal roles in cell differentiation, apoptosis, inflammation and host-pathogen interaction. Furthermore, abnormal concentrations of this glycosidase have been observed in patients suffering from cancer, fucosidosis, rheumatoid arthritis, cystic fibrosis, and leukocyte adhesion deficiency. Industrial applications of α-L-Fucosidase (FUCA1) include synthesis of fucosylated analogs that could serve as antiadhesion compounds, cancer vaccines, and anti-inflammatory therapeutics. BioVision’s α-L-Fucosidase Assay Kit provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples. In this assay, α-L-Fucosidase uses a synthetic chloro p-nitrophenol derivative (R-pNP) as an α-L-Fucosidase substrate and releases a chlorinated pNP derivative which can be measured kinetically under acidic conditions (OD 405 nm). The assay involves a one-step simple reaction, with minimal sample preparation and does not need a stop solution to complete the reaction. The assay can detect as low as 20 µU of α-L-Fucosidase activity in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Biological fluids such as serum, plasma, saliva, etc. |
| 试剂盒组分 | • FUCA1 Assay Buffer • FUCA1 Substrate • pNP Standard (10 mM) • DTT (1 M) • FUCA1 Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |