| 货号 | K336-100 |
| 描述 | Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and PicoProbeTM to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity equivalent to as low as 1.5 µU of p300 in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Immunoprecipitated HAT from cells and tissue • Recombinant enzyme • Nuclear Extract |
| 试剂盒组分 | • HAT Assay Buffer • Acetyl CoA • H4 Peptide • Substrate Mix • Developer • PicoProbe™ • CoA Standard • Positive Control (HeLa Nuclear Extract) |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
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