Microsome Isolation Kit
货号：
K249-50 基本售价：
5100.5 元 规格：
50assays
产品信息
概述| 货号 | K249-50 |
| 描述 | Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily. Microsomal preparations are an affordable and convenient in vitro system for assessing Phase I biotransformation reactions, as they contain all of the xenobiotic-metabolizing CYP isozymes and the membrane-bound flavoenzymes (such as NADPH P450-Reductase and cytochrome b5) required for function of the multicomponent P450 enzyme system. BioVision’s Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | • Applications - Convenient and fast isolation of microsomal fraction from animal tissues- Assessment of CYP-mediated drug metabolism and xenobiotic biotransformation- Protein profiling of microsomal membrane proteins by SDS-PAGE and Western blot |
| 样本类型以及上样量 | • Mammalian glands and soft tissues such as liver, spleen, lungs etc.• Cultured eukaryotic cell lines such as HepG2 human hepatic carcinoma cells |
| 产品特色 | • Simple & convenient protocol• The kit provides unique formulations of ready-to-use buffers and reagents to isolate microsomes without the need for ultracentrifugation or sucrose gradient fractionation |
| 试剂盒组分 | • Homogenization Buffer• Storage Buffer • Protease Inhibitor Cocktail |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
