Glutathione Peroxidase Activity Colorimetric Assay Kit
货号：
K762-100 基本售价：
4979.5 元 规格：
100assays
产品信息
概述| 货号 | K762-100 |
| 描述 | Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes play important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. In BioVision’s Glutathione Peroxidase Activity Assay, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 产品特色 | • Simple procedure; takes ~ 30-40 minutes • Fast and convenient |
| 试剂盒组分 | • GPx Assay Buffer • NADPH (lyophilized)• Glutathione Reductase • Glutathione (GSH, lyophilized)• Cumene Hydroperoxide • GPx Positive Control (lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
