cAMP Direct Immunoassay Kit (Colorimetric)
货号：
K371-100 基本售价：
4979.5 元 规格：
100assays
产品信息
概述| 货号 | K371-100 |
| 描述 | Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. BioVision’s cAMP Assay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 μl (or ~ 0.02-2 μM) cAMP samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The kit can detect ~0.1-10 pmol/5 μl (or ~ |
| 产品特色 | • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • The kit provides a new acetylation procedure to improve detection sensitivity significantly |
| 试剂盒组分 | • 10X cAMP Assay Buffer • Standard cAMP (10 nmol) • Neutralizing Buffer • Acetylating Reagent A • Acetylating Reagent B • Rabbit Anti-cAMP pAb • HRP Developer • Protein G Coated Plate |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
