Dihydrofolate Reductase Activity Kit (Colorimetric)
货号：
K246-100 基本售价：
4979.5 元 规格：
100assays
产品信息
概述| 货号 | K246-100 |
| 描述 | Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC 1.5.1.3), is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells. It catalyzes the reduction of dihydrofolate (FH2) to tetrahydrofolate (FH4) using NADPH as a cofactor. FH4 is essential for a number of enzymes that are necessary for the de novo synthesis of purines, thymidylic acid and some amino acids. Inactivation of the DHFR enzymatic activity causes reduction of the intracellular level of FH4, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important enzyme as a molecular target in drug discovery. Biovision’s Dihydrofolate Reductase assay kit is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96- well plate. The assay is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD=340 nm)• Species reactivity - Mammalian• Applications - Detection of Dihydrofolate Reductase activity |
| 样本类型以及上样量 | • Purified recombinant protein• Cell and tissue lysate |
| 产品特色 | • Rapid, sensitive and convenient• The kit measures total Activity of DHFR Activity with limit of quantification 4 mU/ml using recombinant DHFR |
| 试剂盒组分 | • DHFR Assay Buffer• DHFR Substrate• Dihydrofolate Reductase• NADPH• |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
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