Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)
货号：
K205-100 基本售价：
4979.5 元 规格：
100assays
产品信息
概述| 货号 | K205-100 |
| 描述 | Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 µU of NOS activity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Colorimetry (Abs: 540 nm)• Species reactivity - Mammalian samples• Applications - Detection of NOS activity |
| 样本类型以及上样量 | • Purified recombinant protein • Tissue or cell extracts |
| 产品特色 | • Simple, HTP adaptable protocol• Sensitivity as low as 5 µU of NOS activity• Colorimetric detection |
| 试剂盒组分 | • NOS Assay Buffer • NOS Dilution Buffer • NOS Substrate• NOS Cofactor 1 • NOS Cofactor 2 (25X)• Nitrate Reductase• NOS (Positive Control)• Enhancer• Nitrite Standard• Griess Reagent 1• Griess Reagent 2 |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
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