Aconitase Activity Colorimetric Assay Kit
货号：
K716-100 基本售价：
4858.5 元 规格：
100assays
产品信息
概述| 货号 | K716-100 |
| 描述 | Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc. • Species reactivity- Mammalian |
| 产品特色 | • Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Aconitase activity in various biological samples. |
| 试剂盒组分 | • Assay Buffer • Substrate • Developer • Enzyme Mix (200µl) • Cysteine • (NH₄)₂Fe(SO₄)₂ • Isocitrate Standard (10µmol) |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
