货号 | K364-100 |
描述 | The coagulation Factor IX (or Christmas factor) (EC 3.4.21.22) is a vitamin K-dependent serine protease. Factor IX is produced as an inactive precursor and is activated via cleavage by either factor XIa (contact pathway) or factor VIIa (tissue factor pathway). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor IX (FIXa) then binds to the activated Factor VIII (FVIIIa) and proteolytically activates factor X (FX) to factor Xa (FXa). BioVision’s Factor IXa Activity Assay kit is based on the ability of FIXa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 10 pg of FIXa in a variety of samples. |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
供应商 | Biovision |
样本类型以及上样量 | • Purified Factor Ixa, Plasma, Serum |
试剂盒组分 | • FIXa Assay Buffer • FXa Substrate-AMC • Enzyme Mix I • Enzyme Mix II • Phospholipids • FIXa Enzyme Standard (10 ng) |
运输条件 | Blue Ice |
存放说明 | -20℃ |