Nitric Oxide Fluorometric Assay Kit
货号：
K252-200 基本售价：
4812.5 元 规格：
2x96assays
产品信息
概述| 货号 | K252-200 |
| 描述 | Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 380/461 nm) • Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- Measurement of total nitrate/nitr |
| 产品特色 | • Simple procedure; takes only 2-3 hours • Fast and convenient • The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate. |
| 试剂盒组分 | • Assay Buffer • Enzyme Cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • DAN Probe • Sodium Hydroxide • Microtiter plate • Plate Cover |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
