Isocitrate Dehydrogenase Activity Colorimetric Assay Kit
货号：
K756-100 基本售价：
4689.5 元 规格：
100assays
产品信息
概述| 货号 | K756-100 |
| 描述 | Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NADᶧ) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing α-ketoglutarate and CO2 while converting NADᶧ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, α-ketoglutarate. Other isoforms (EC 1.1.1.42, NADPᶧ) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADPᶧ as a cofactor instead of NADᶧ. BioVision’s Isocitrate Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of NADᶧ-dependent, NADPᶧ-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc. • Species reactivity- Mammalian • Application- This assay has a detection sensitivity as |
| 产品特色 | • Simple procedure • Fast and convenient • Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses. |
| 试剂盒组分 | • IDH Assay Buffer • NADᶧ (lyophilized) • NADPᶧ (lyophilized) • IDH Substrate (lyophilized) • Developer (lyophilized) • IDH Positive Control (NADPᶧ) • NADH Standard (0.5 μmol, lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
