Sphingomyelin Quantification Colorimetric Assay Kit
货号：
K600-100 基本售价：
4689.5 元 规格：
100assays
产品信息
概述| 货号 | K600-100 |
| 描述 | Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD 570 nm)• Species reactivity - N/A• Applications:-Sphingomyelin quantification in various biological samples- Analysis of cell signaling pathway |
| 样本类型以及上样量 | • Serum, plasma• Tissue samples such as brain etc.• Adhesion or suspension cells |
| 产品特色 | • Simple, rapid & convenient• The assay can detect sphingomylein less than 1 µM in a variety of samples |
| 试剂盒组分 | • SM Assay Buffer • OxiRed Probe™• Sphingomyelinase (Lyophilized)• ALP Enzyme (Lyophilized)• SM Enzyme Mix (Lyophilized)• SM Standard (5 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
