Proteasome Activity Fluorometric Assay Kit
货号：
K245-100 基本售价：
4689.5 元 规格：
100assays
产品信息
概述| 货号 | K245-100 |
| 描述 | Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes). They are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. BioVision’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 350/440nm)• Sample type- Whole cell lysates, nuclear and cytoplasmic lysates• Species reactivity- Mammalian• Applications- Specifically measures proteasome activity from other protease activity present in samples.. |
| 产品特色 | • Simple one-step procedure; takes only 30-60 minutes• Accurate and easy to use |
| 试剂盒组分 | • Proteasome Assay Buffer• Proteasome Substrate (In DMSO)• Proteasome Inhibitor (In DMSO)• AMC Standard (1 mM in DMSO)• Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
