Sphingomyelinase Activity Fluorometric Assay Kit
货号：
K574-100 基本售价：
4628.0 元 规格：
100assays
产品信息
概述| 货号 | K574-100 |
| 描述 | Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. BioVision’s SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 µU/ml in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 535/587 nm)• Applications - Measurement of SMase activity in various tissues/cells extracts |
| 样本类型以及上样量 | • Animal tissues: brain, heart, kidney, etc.• Cell culture: Adherent or suspension cells |
| 产品特色 | • Simple, rapid & convenient• The assay can detect sphingomyleinase activity as low as 10 µU/ml in a variety of samples |
| 试剂盒组分 | • SMase Assay Buffer• SMase Probe (DMSO)• SMase Substrate (Lyophilized)• SMase Enzyme Mix I (Lyophilized)• SMase Enzyme Mix II (Lyophilized)• Choline Standard (Lyophilized)• SMase Positive Control (Lyophilized)• SMase Extraction Detergent |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
