GFP Quantitation Kit
货号：
K815-100 基本售价：
4137.5 元 规格：
100assays
产品信息
概述| 货号 | K815-100 |
| 描述 | Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea Victoria (Chalfie et al, 1994). GFP has also been recombinant modified and enhanced (called EGFP). GFP and EGFP have been widely used as a valuable reporter molecule for in vivo visualization of gene expression events in a variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP fluorescence can be easily detected under fluorescence microscope. However, most imaging studies of GFP are only qualitative. Since quantitative analysis of GFP expression level in cells or tissues are more informative and have wide applications, BioVision has developed the GFP Quantification Kit which quantifies GFP in 96 micro-plate format. Cells or tissues can be homogenized directly in the GFP Assay Buffer. The quantity of GFP is determined by comparing its fluorescence with that of GFP standard. The kit can detect a wide range of GFP concentration (0.01-10 µg/ml). A GFP quench solution is also provided for determining auto-fluorescence of cell or tissue extracts. Each kit provides sufficient reagents to perform up to 100 assays, Ex/Em = 488/507 nm. |
| 同种亚型 | Rabbit IgG1 |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 488/507 nm) • Sample type- Cell and Tissue culture • Species reactivity- Mammalian • Application- It detects a wide range of GFP concentration (0.01-10 ug/ml) |
| 产品特色 | • Simple procedure; takes ~ 20 minutes • Fast and convenient |
| 试剂盒组分 | • GFP Assay Buffer • GFP Standard (100 µg) • GFP Quench Solution |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
