Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)
货号：
K549-100 基本售价：
4137.5 元 规格：
100assays
产品信息
概述| 货号 | K549-100 |
| 描述 | Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are observed during inflammation. Pharmacological inhibition of COX by non-steroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain. BioVision’s COX Activity Assay Kit provides a simple, sensitive, and high-throughput adaptable method to detect the peroxidase activity of COX in biological samples or purified/crude enzyme preparations. The kit includes COX-1 and COX-2 specific inhibitors to differentiate the activity of COX-1 and COX-2 as well as other peroxidases, which may be present in the sample. Detection limit: 6 μU/mg. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 535/587 nm) • Applications - Measurement of COX activity in various biological samples and purified/crude enzyme preparations |
| 样本类型以及上样量 | • Adherent and suspension cells• Animal tissues such as rat liver • Purified enzyme |
| 产品特色 | • Simple, rapid & convenient assay to measure both COX-1 and COX-2 activity • High-throughput adaptable• Includes COX-1 inhibitor, SC560 & COX-2 Inhibitor, Celecoxib |
| 试剂盒组分 | • COX Assay Buffer• COX Probe (in DMSO)• COX Cofactor (in DMSO)• Arachidonic Acid• NaOH• COX-1 Positive Control • Resorufin Standard (5 mM, in DMSO)• SC560 (COX-1 inhibitor in DMSO) • Celecoxib (COX-2 inhibitor in DMSO) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
