| 货号 | K383-100 |
| 描述 | Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, elastase 2, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it causes degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. Extracellular elastase is implicated in the signs, symptoms and disease progression of inflammatory lung disorders (such as cystic fibrosis, COPD, lung emphysema) via its role in the inflammatory processes, mucus overproduction and lung tissue damage. BioVisions Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence microplate readers. This assay kit is simple, rapid and can detect as low as 1 ng of Neutrophil Elastase in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Purified enzyme • Blood, plasma |
| 试剂盒组分 | • NE Assay Buffer • NE Dilution Buffer • NE Substrate • NE Enzyme Standard (1 μg) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |