PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit
货号：
K314-500 基本售价：
4137.5 元 规格：
500assays
产品信息
概述| 货号 | K314-500 |
| 描述 | Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. BioVision’s PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm)• Applications - Measurement of released LDH in culture medium• Evaluation of cytotoxicity effect of test compounds |
| 样本类型以及上样量 | • Adherent or Suspension Cells |
| 产品特色 | • Highly-Sensitive: Detects as less as 100 cells.• Rapid protocol: requires less than 20 min.• High-throughput adaptable. |
| 试剂盒组分 | • LDH Assay Buffer• LDH Substrate Mix • PicoProbeTM • Cell Lysis Solution• LDH Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
