EZCell™ Glutathione Detection Kit (Blue Fluorescence)
货号：
K504-100 基本售价：
3887.5 元 规格：
100assays
产品信息
概述| 货号 | K504-100 |
| 描述 | Glutathione (GSH) represents major low molecular-weight free thiol in living cells. As the key antioxidant in mammalian cells, intracellular GSH forms rapidly eliminated conjugates with electrophilic xenobiotics, free radicals as well as hydroperoxides. Insufficiency of cellular GSH results in oxidative stress damage and mitochondrial degeneration. Diminished levels of glutathione are symptomatic of cell stress thus; it is advantageous to monitor distribution of the intracellular GSH as an indicator of early apoptosis and cell death. BioVision’s EZCellTM Glutathione Detection Kit (Blue Fluorescence) utilizes Monochlorobimane (MCB), a non-fluorescent and cell-permeable dye, that forms highly fluorescent adducts with GSH (GSH-MCB) detectable at EX/EM= 380/465 ±20 nm respectively. The reaction is catalyzed by glutathione-S-transferase (GST) and the intensity of the fluorescence reflects the amount of intracellular GSH. Hence, our kit provides a useful tool for fast and easy evaluation of the GSH effectors directly in the living cells. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 380/460 nm)• Species reactivity - • Applications - Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells- Screening for compounds affecting intracellular GSH levels |
| 样本类型以及上样量 | • Live cell cultures (adherent or suspension) |
| 产品特色 | • Highly sensitive fluorometric method to measure intra-cellular Glutathione levels in response to a variety of biochemical stimuli• Simple & High throughput-adaptable• Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Glutathione metabolism |
| 试剂盒组分 | • Wash Buffer• Monochlorobimane (MCB) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
