LDH-Cytotoxicity Colorimetric Assay Kit
货号：
K311-400 基本售价：
3762.5 元 规格：
400assays
产品信息
概述| 货号 | K311-400 |
| 描述 | Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5–1 hr. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (500 nm) • Sample type- Cell culture (adherent and suspension) • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release a |
| 产品特色 | • Simple one-step procedure; takes less than 1 hour • Fast, sensitive and convenient |
| 试剂盒组分 | • Catalyst, Lyophilized • Dye Solution |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
