| 货号 | NBP2-29482 |
| 供应商 | Novus |
| 背景 | The Endoplasmic Reticulum (ER) is a membranous labyrinth that extends throughout the cytoplasm of the cell as a single continuous network of flattened sacs and tubules. The ER accounts for more than half the total membrane in eukaryotic cells and 10% of a cells total volume, thus representing one of the largest organelles. It plays a critical role not only in lipid and protein biosynthesis but is central to protein modification, folding, and assembly. The ER is generally characterized as being devoid of membrane-bound ribosomes, (smooth ER), or studded with ribosomes (rough ER); each differing in structure, appearance, and function. Although isolating the ER may seem difficult due to its size and complex association with other components of the cytosol, the Endoplasmic Reticulum Enrichment Kit is a key tool that provides the necessary reagents together with an optimized protocol required to gently isolate and enrich for ER from tissues. |
| 存放说明 | Storage is content dependent. |
Endoplasmic Reticulum Enrichment Assay Kit [NBP2-29482] | |
Endoplasmic Reticulum Enrichment Extraction Kit [NBP2-29482] - This kit is routinely tested and standardized using 0.5 gram of mouse liver tissue. | |
Endoplasmic Reticulum Enrichment Extraction Kit [NBP2-29482] - Analysis of ATF6 (3 ug/ml) and GAPDH (0.25 ug/ml). ATF6 is an ER stress-regulated transmembrane transcription factor. The blots were probed with ATF6, stripped and reprobed with GAPDH. Full-length ATF6 was detected in all 3 lanes, and was enriched in the ER fractions. GAPDH was detected in the whole lysate and total ER fraction, but not in the rough ER fraction. ATF6* may represent partial or cleaved/active ATF6. |
