TMRE Mitochondrial Membrane Potential Assay Kit (Fluorometric)
货号：
K238-200 基本售价：
3637.5 元 规格：
200assays
产品信息
概述| 货号 | K238-200 |
| 描述 | Mitochondria are the powerhouse of the cells and generate energy in the form of ATP. Mitochondria utilize oxidizable substrates to produce a membrane potential in the form of a proton gradient across the mitochondrial inner membrane. Mitochondrial membrane potential is tightly interlinked to many mitochondrial processes, including ATP synthesis, ROS, calcium sequestration and import of proteins into the mitochondrion. Therefore, pharmacological changes in mitochondrial membrane potential are associated with several pathological parameters. BioVision’s TMRE Mitochondrial Membrane Potential Assay Kit uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell-permeable, positively-charged, red-orange dye that readily accumulates in active mitochondria. Protonophores like FCCP and CCCP induces dissipation of mitochondrial membrane thus results in compromised staining. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm), FACS [FL-3 (Red) channel], Fluorescent microscopy [red filter (Ex/Em = 549/575 nm)]• Applications - Labeling of active mitochondria in cells- Screening of compounds which may compromise mitochondrial membrane po |
| 样本类型以及上样量 | • Adherent and suspension cells |
| 产品特色 | • Convenient method• Based on cell-permeable, positively-charged, red-orange dye TMRE, that readily accumulates in the active mitochondria• Includes Protonophores FCCP to validate the assay |
| 试剂盒组分 | • Assay Buffer• TMRE Dye (1 mM, in DMSO)• Negative Control (FCCP, 40 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
