Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)
货号：
K315-100 基本售价：
3262.5 元 规格：
100assays
产品信息
概述| 货号 | K315-100 |
| 描述 | Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (FL1 channel and FL3 channel)• Applications - Measurement of cell cytotoxicity in response to drug or toxin treatment• Quantification of the cytotoxic effect of immune effector cells on target cells• Assessment of physiol |
| 样本类型以及上样量 | • Adherent and suspension cells |
| 产品特色 | • Direct method to measure cell-mediated cytotoxicity• Does not require cell lysis• Provides multi-parametric analysis |
| 试剂盒组分 | • Cytotoxicity Assay Buffer• 7-AAD Staining Solution• CFSE Staining Solution |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
