BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible
货号：
K819-250 基本售价：
3137.5 元 规格：
250assays
产品信息
概述| 货号 | K819-250 |
| 描述 | BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This BCA protein assay kit is adapted from BioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determining protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over the widest range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The BCA protein assay kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples. |
| 克隆号 | 4G6E11 |
| 同种亚型 | Mouse IgG1 |
| 来源宿主 | Mouse |
性能| 供应商 | Biovision |
| 样本类型以及上样量 | • pure proteins, cell/tissue extracts or lysates |
| 产品特色 | • Simple & convenient.• Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM).• The BCA protein assay is linear over the widest range of protein concentration (25-2000 μg/ml). |
| 试剂盒组分 | • BCA Reagent A• BCA Reagent B• BSA Standard (2 mg/ml)• Blocking Reagent (20 mg/tube)• Blocking Reagent Buffer |
| 运输条件 | Blue Ice |
| 存放说明 | RT |
