Irradiated Mouse Embryonic Fibroblasts (5 vials) (1 PK)
货号：
PSC001 基本售价：
2825.6 元 规格：
1 Pack
产品信息
概述| 货号 | PSC001 |
| 简介 | 6 x 10e6 cells/vial |
| 别名 | Embryonic Stem Cells |
性能| 供应商 | R&D Systems |
| 试剂盒组分 | Five vials of irradiated mouse embryonic fibroblasts are provided with 6 x 106 cells in each vial. Mouse embryonic fibroblasts (MEF) were isolated from E13.5 CF1 embryos after removal of brain and visceral tissue. The MEF were then mitotically inactivated at passage 3 by gamma irradiation and cryopreserved. Stability and StorageStore in liquid nitrogen for up to 2 years. Quality ControlR&D Systems iMEF are tested for their ability to support undifferentiated growth of BG01V human ES cells as assessed by Oct-4 and SSEA-4 expression. They are mycoplasma negative as tested by the MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B) and negative for microbial contamination. PrecautionsThis product contains 10% dimethylsulfoxide (DMSO). Data Examples | Human Pluripotent Stem Cells Cultured on Mouse Embryonic Fibroblasts Express Pluripotent Markers SSEA-4, Oct-3/4, Oct-4A, and E-Cadherin. A. Human iPS2 stem cells were cultured on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001). Expression of SSEA-4 was detected using a Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) followed by the NorthernLights™ (NL) 493-conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL009, green). Oct-3/4 expression was detected using a Goat Anti-Human Oct-3/4 Affinity Purified Polyclonal Antibody (Catalog # AF1759) followed by the NL557-conjugated Donkey Anti-Goat IgG (Catalog # NL001, red). The nuclei were counterstained with DAPI (blue). B. BG01V human embryonic stem cells were cultured on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001). Expression of Oct-4A was detected using a Mouse Anti-human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by the NL493-conjugated Donkey Anti-mouse IgG Secondary Antibody (Catalog # NL009, green). Expression of E-Cadherin was detected using a Goat Anti-Human E-Cadherin Affinity Purified Polyclonal Antibody (Catalog # AF648) followed by the NL 557-conjugated Donkey Anti-Goat IgG (Catalog # NL001, red). Nuclei were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
| BG01V Human Embryonic Stem Cells Cultured on Irradiated Mouse Embryonic Fibroblasts Demonstrate a Phenotype that is Consitent with Pluripotency. A. BG01V human embryonic stem cell colonies cultured on Irradiated Mouse Embryonic Fibroblasts (Catalog # PSC001)were analyzed for markers of pluripotency after 3 passages. B. Cells were evaluated for SSEA-4 expression by flow cytometry using a PE-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (Catalog # FAB1435P; filled histogram) or a PE-conjugated Mouse IgG3 Isotype Control Antibody (Catalog # IC007P; open histogram). C.&D. BG01V human embryonic stem cell colonies were incubated with a Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or a Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (Catalog # NL001). Expression of SSEA-4, Oct-3/4, and Nanog is consistent with a pluripotent phenotype. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
BG01V human embryonic stem cells are licensed from ViaCyte, Inc. Preparation and Storage |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A comparative study of protocols for mouse embryonic stem cell culturing.
Authors: Tamm C, Pijuan Galito S, Anneren C
PLoS ONE, 2013;8(12):e81156. 2013
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| 运输条件 | 多种成分多种运输条件 |
| 存放说明 | 多种成分不同储存条件 |
| 参考文献 | - Human Oncostatin M and Mouse LIF Support Mouse ES Cell Pluripotency & Germline Competency
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