beta Galactosidase IHC Kit
货号：
NBP2-29546 基本售价：
2815.7 元 规格：
1 Kit
产品信息
性能| 供应商 | Novus |
| 免疫原 | B-Gal Staining Kit for the Detection of Beta-Galactosidase (B-Gal) enzyme in transfected Cells |
| 背景 | The LacZ gene encodes for the Beta-Galactosidase (Beta-Gal) enzyme that catalyzes the hydrolysis of B-galactosides, including lactose. The LacZ gene is among the most widely used and published reporter genes due in large part to the extreme resistance of the B-Gal enzyme to proteolytic degradation. B- Gal enzyme activity is also readily detected in stably or transiently transfected cells or transgenic tissues using a variety of substrates B-Gal Staining Kit is based on 5-bromo-4-chloro-3-indolyl-B-Dgalactopyranoside (X-Gal), a noninducing chromogenic substrate for B-Gal. When B-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl monomer is produced. Subsequently, two of the liberated indoxyl moieties form a dimer which is nonenzymatically oxidized. The dimerizatation and oxidation reactions require transfer of an electron which is facilited, in this case, by ferric and ferrous ions. The resultant halogenated indigo is a very stable and insoluble dark blue compound (Fig ). Following transfection with plasmids that contain LacZ, and the addition of X-Gal, the transfection efficiency can be determined by counting the number of blue cells in the total cell population(Fig) |
| 存放说明 | Storage is content dependent. |
参考图片Western Blot: beta Galactosidase IHC Kit [NBP2-29546] - 5-bromo-4-chloro-3-indolyl-Beta-D-galactopyranoside (X-Gal) Chromogeneic substrate. Beta-Gal cleavage of the glycosidic linkage in X-Gal (1) produces a colorless indoxyl monmer, 5-bromo-4-chloro-indolyl (2). Nonenzymatic oxidation and dimerization occur in the presence of ferric and ferrous ions resulting in 5,5-di-bromo-44-dichloro-indigo, a stable and insoluble dark blue halogenated indigocompound (3). |
