Cholesterol Detection Kit (cell-based)
货号：
K587-100 基本售价：
2762.5 元 规格：
100assays
产品信息
概述| 货号 | K587-100 |
| 描述 | Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compartments is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm). • Applications - Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells. |
| 样本类型以及上样量 | • Cells |
| 产品特色 | • Simple, easy to perform, histochemical method of identification of unesterified cholesterol. • Includes U-18666A, a cholesterol transport inhibitor. |
| 试剂盒组分 | • Fixative Solution • Assay Buffer • Staining Dye • Inhibitor (U-18666A, 2.5 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
