Cell Fractionation Kit【科瑞奥博】

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Cell Fractionation Kit

货号： 9038S 基本售价： 2644.0 元规格： 1Kit

产品信息

概述
货号	9038S
描述	The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.
反应种属	Human
应用	W/FUNC

性能
供应商	CST
背景	Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
存放说明	-20C
参考文献	1 . Lenstra, J.A. and Bloemendal, H. (1983) Eur J Biochem 135, 413-23. 2 . Banno, A. et al. (2012) J Biol Chem 287, 13799-812. 3 . Loo, L.H. et al. (2009) J Cell Biol 187, 375-84. 4 . Michelsen, U. and von Hagen, J. (2009) Methods Enzymol 463, 305-28.

参考图片

Western blot analysis of cell fractions from HeLa cells using MEK1/2 (D1A5) Rabbit mAb #8727, AIF (D39D2) XP® Rabbit mAb #5318, Histone H3 (D1H2) XP® Rabbit mAb #4499, and Vimentin (D21H3) XP® Rabbit mAb #5741 (as part of the Cell Fractionation Antibody Sampler Kit #11843) showing cytoplasmic, organellular/membrane, and nuclear/cytoskeletal localization. Whole cell lysates (WCL) were used to represent total protein. Cytoplasmic proteins (Cyto) were isolated using CIB buffer. Integral membrane and organellular proteins (Mem) were isolated using MIB buffer. Nuclear and cytoskeletal proteins (Nuc) were isolated using CyNIB buffer.Western blot分析HeLa细胞的分离物来显示细胞质、细胞膜/细胞器及细胞核/细胞骨架的定位，使用的抗体是：MEK1/2 (D1A5) Rabbit mAb #8727、AIF (D39D2) XP® Rabbit mAb #5318 Histone H3 (D1H2) XP® Rabbit mAb #4499和Vimentin (D21H3) XP® Rabbit mAb #5741 (as part of the Cell Fractionation Antibody Sampler Kit #11843)。全细胞裂解物（WCL）代表总蛋白质。细胞质的蛋白（Cyto）用CIB缓冲液分离。完整的膜和细胞器的蛋白（Mem）用MIB缓冲液分离。细胞核和细胞骨架的蛋白（Nuc）用CyNIB缓冲液分离。