Thioredoxin Reductase Colorimetric Assay Kit
货号：
10007892-96Well 基本售价：
2492.0 元 规格：
96 wells
产品信息
概述| 货号 | 10007892-96Well |
| 描述 | Cayman’s Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm.1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.3 |
| 别名 | TrxR; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Smith, A.D.,Morris, V.C., and Levander, O.A. Rapid determination of glutathione peroxidase and thioredoxin reductase activities using a 96-well microplate format: Comparison to standard cuvette-based assays. International Journal for Vitamin and Nutrition Research 71(1), 87-92 (2001).
2.Hill, K.E.,McCollum, G.W., and Burk, R.F. Determination of thioredoxin reductase activity in rat liver supernatant. Analytical Biochemistry 253, 123-125 (1997).
3.Smith, A.D.,Guidry, C.A.,Morris, V.C., et al. Aurothioglucose inhibits murine thioredoxin reductase activity in vivo. Journal of Nutrition 129, 194-198 (1999).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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