Secreted Alkaline Phosphatase Reporter Assay Kit
货号：
NBP2-25285 基本售价：
2082.0 元 规格：
1 Kit
产品信息
性能| 供应商 | Novus |
| 免疫原 | This Kit provides sufficient reagents for 400 quantitative measurements of SEAP protein in 96-well microtiter format. All 96 wells can be used at one time or you may use only the wells as needed by your experimental design. Use of duplicate wells for the SEAP standard is recommended to obtain accurate results. This kit can be used with supernatant from transfected cells as described or serum as determined by your experimental protocol. |
| 背景 | Placental alkaline phosphatase (PLAP) is one of the most stable isoenzymes, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of PLAP is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is designed by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein SEAP. |
| 存放说明 | Storage is content dependent. |
