| 货号 | 10009325-1ea |
| 描述 | Cayman’s Malachite Green Phosphate Assay Kit provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm.1,2 Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates.3 This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications.4 The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.DAngelo, E.,Crutchfield, J., and Vandiviere, M. Rapid, sensitive, microscale determination of phosphate in water and soil. Journal of Environmental Quality 30, 2206-2209 (2001). 2.OToole, M.,Lau, K.T.,Shepherd, R., et al. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector. Analytica Chimica Acta 597, 290-294 (2007). 3.Maehama, T.,Taylor, G.S.,Slama, J.T., et al. A sensitive assay for phosphoinositide phosphatases. Analytical Biochemistry 279, 248-250 (2000). 4.Attin, T.,Becker, K.,Hannig, C., et al. Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions. Clinical Oral Investigations 9(3), 203-207 (2005). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | 4 |
| 稳定性 | ≥ 1 year |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |