| 货号 | 10821S |
| 供应商 | CST |
| 背景 | A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within recurring sequences, or motifs, in target proteins. These are not limited to consensus sequences that are substrates for protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). Phosphoprotein interacting domains are critical elements in regulating intracellular communication. For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site preceded by Phe at -4 and -1 and followed by Tyr at +1 (3). Phosphorylation at this and similar sites on RSK2, p70 S6 kinase and certain PKC isoforms is required for their binding to 3-phosphoinositide-dependent kinase 1 (PDK1) and subsequent downstream signaling (4-7). Through these interactions, PDK1 is involved in the regulation of key cellular processes including proliferation, differentiation and apoptosis. Cell Signaling Technology has developed phospho-Ser/Thr motif antibodies as powerful tools for proteomic profiling of kinase substrates and phosphoprotein binding domains. |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 参考文献 | 1 . Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225. 2 . Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8. 3 . Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 4 . Frödin, M. et al. (2000) EMBO J 19, 2924-34. 5 . Balendran, A. et al. (1999) J Biol Chem 274, 37400-6. 6 . Balendran, A. et al. (2000) J Biol Chem 275, 20806-13. 7 . Vanhaesebroeck, B. and Alessi, D.R. (2000) Biochem J 346 Pt 3, 561-76. |
This chart shows the underlying motif distribution in a PhosphoScan® LC-MS/MS experiment using 180 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 (10 nM, 20 min) and Pervanadate (1 mM, 20 min) and immunoprecipitated with PTMScan® Phospho-PDK1 Docking Motif [(F/Y)p(S/T)(F/Y)] Immunoaffinity Beads. | |
The motif logo was generated from a PhosphoScan® LC-MS/MS experiment of 180 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 (10 nM, 20 min) and Pervanadate (1 mM, 20 min) and immunoprecipitated with PTMScan® Phospho-PDK1 Docking Motif [(F/Y)p(S/T)(F/Y)] Immunoaffinity Beads. The logo demonstrates the relative abundance of amino acids in a given position around the centrally phosphorylated residue within this data set. |
