PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit【科瑞奥博】

产品中心

当前位置：首页>产品中心

PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit

货号： 32948S 基本售价：询价规格： 1Kit

产品信息

概述
货号	32948S

性能
供应商	CST
背景	As an integral part of the machinery of cellular function, proteins undergo regulation by a variety of post-translational modifications. One of the most prevalent and widely studied PTMs is serine/threonine phosphorylation. Prominent kinases targeting consensus substrate motifs account for tens of thousands of known and predicted sites on more than 13,000 human proteins (1-3). Cell Signaling Technology has developed phospho-Ser/Thr motif antibodies for proteomic profiling of kinase substrates. Arg-directed or AGC-family kinases including PKA, PKG, PKC, Akt, p70 S6 kinase, AMPK, and RSK and are characterized by preference for basic amino acids (Lys or Arg) especially Arg at position -3 relative to the phosphorylated Ser or Thr (1,4). Akt, p70S6 kinase and RSK additionally share specificity for Lys or Arg at position -5 (5). CST™ has developed a Basophilic PTMScan^® kit comprising a pool of antibodies that recognize basophilic kinase substrate motifs as a powerful proteomics analysis tool for investigating the regulation of phosphorylation by Arg-directed kinases, as well as for high throughput kinase drug discovery.
运输条件	0.75
存放说明	-20C
参考文献	1 . Rao, R.S. and Møller, I.M. (2012) Biochim Biophys Acta 1824, 405-12. 2 . Goel, R. et al. (2012) Mol Biosyst 8, 453-63. 3 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 4 . Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 5 . Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74.

参考图片

The Motif Logo was generated from a PhosphoScan^® LC-MS/MS experiment using 1526 nonredundant Lys-C digested peptides derived from mouse embryo immunoprecipitated with PTMScan^® Basophilic Kinase Motif [(R/X)(R/X)Xp(S/T)] Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position surrounding the central phosphorylated residue.

This chart shows the underlying motif ditribution in a PhosphoScan^® LC-MS/MS experiment using 1526 nonredundant Lys-C digested peptides generated from mouse embryo and immunoprecipitated using PTMScan^® Basophilic Kinase Motif [(R/X)(R/X)Xp(S/T)] Immunoaffinity Beads. Phospho-threonine peptides comprised 17% of peptides in this data set while phospho-serine contributed 83%.