PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit【科瑞奥博】

产品中心

当前位置：首页>产品中心

PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS/T) Kit

货号： 5563S 基本售价：询价规格： 1Kit

产品信息

概述
货号	5563S

性能
供应商	CST
背景	Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (2-4). It is a member of an important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, which includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, p70S6 kinase, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (5,6). Akt, p70 S6 kinase, and RSK additionally share specificity for Arg at position -5 (recognition sequence RXRXXS/T) (7) In a recent phosphoproteomic study (8) co-authored by scientists in the CST Site Discovery Group over 300 downstream substrates for AGC family kinases recognizing the RXRXXS/T motif were identified with PhosphoScan Technology using Phospho-Akt substrate antibodies. These CST™ antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. In this assay, PTMScan^® (RXRXXS/T) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the (RXRXXS/T) motif (S* = phospho-serine, T* = phospho-threonine).
存放说明	-20C
参考文献	1 . Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3 . Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74. 4 . Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8. 5 . Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53. 6 . Moritz, A. et al. (2010) Sci Signal 3, ra64. 7 . Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4. 8 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

参考图片

Chart showing the proportions of underlying Akt substrate-like sequence motifs found in a PTMScan® study using Akt substrate motif mAb 2 and Orbitrap LC-MS analysis. Analysis of one experiment on peptides from H3255 cells gave 121 non-redundant sites containing Akt substrate-like motifs. The primary motifs are highlighted in white. The proportion containing phosphoserine was 72%, and the proportion with arginine residues at both the -5 and -3 positions was 65%. This antibody has a strong preference for arginine residues at the -5 and -3 positions, and to a lesser extent recognizes peptides with an arginine residue at just the -3 position.

图片显示使用Akt底物模体mAb 2和Orbitrap LC-MS分析进行的PTMScan研究中发现的Akt底物样序列模体的属性。对H3255细胞肽段的实验的分析给出包含Akt底物样模体的121个非冗余位点。主要模体用白色加亮显示。包含磷酸化丝氨酸的比率为72%，在-5和-3位有精氨酸残基的比率为65%。尽管此抗体对-5和-3位有精氨酸的模体有偏好，它也能以较弱程度识别仅在-3位有精氨酸残基的肽段。

The Motif Logo shows the amino acid distributions around the sites recognized by the antibody.

模体标志图显示出分布在此抗体识别位点周围的氨基酸。

Akt substrate motif western blot analysis of extracts from MKN45 cells, untreated or Met inhibitor Su11274-treated, usingPhospho-Akt Substrate Motif (RXRXXS*/T*) Rabbit mAb.

使用Phospho-Akt Substrate Motif (RXRXXS*/T*) Rabbit mAb对来自未处理或Met抑制剂Su11274处理的MKN45细胞抽提液进行Akt底物模体的Western Blot分析。