| 货号 | 5561S |
| 供应商 | CST |
| 背景 | Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (2-4). It is a member of an important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, which includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, p70 S6 kinase, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (5,6). Akt, p70S6 kinase and RSK additionally share specificity for Arg at position -5 (recognition sequence RXRXXS/T) (7) In a recent phosphoproteomic study (8) co-authored by scientists in the CST Site Discovery Group over 300 downstream substrates for AGC family kinases recognizing the RXRXXS/T motif were identified with PhosphoScan Technology using Phospho-Akt substrate antibodies. These CST™ antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. In this assay, PTMScan® (RXXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phospho-peptides containing the RXXS*/T* motif (S*= phospho-serine, T*= phospho-threonine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3 . Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74. 4 . Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8. 5 . Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53. 6 . Moritz, A. et al. (2010) Sci Signal 3, ra64. 7 . Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4. 8 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportions of underlying Akt substrate-like sequence motifs found in an Akt substrate PTMScan® study using Akt Substrate Motif mAb 1. Analysis of three cell lines gave 281 non-redundant peptide sequences containing Akt substrate-like motifs (Moritz et al., Akt-RSK-S6 kinase signaling networks activated by oncogenic receptor tryosine kinases. Sci. Signal. 2010, 3, ra64). The proportion containing phosphoserine was 74%, and the proportion with arginine residues are both the -5 and -3 positions was 36%. Although this antibody has a preference for arginine residues at the -5 and -3 positions, it also recognizes peptides with an arginine residue at just the -3 position. 图片显示使用Akt Substrate Motif mAb 1进行的AKt底物PTMScan研究中发现的Akt底物样序列模体的属性. 对着三个细胞系的分析给出了281个包含Akt底物样模体的非冗余肽段序列（Moritz等，Akt-RSK-S6激酶信号通路网络被致癌基因受体酪氨酸激酶激活，Sci. Signal. 2010, 3, ra64）。包含磷酸化丝氨酸的比率为74%，在-5和-3位有精氨酸残基的比率为36%。尽管此抗体对-5和-3位有精氨酸的模体有偏好，它也能识别仅在-3位有精氨酸残基的肽段。 | |
The Motif Logo shows the amino acid distributions around the sites recognized by the antibody. 模体标志图显示出分布在此抗体识别位点周围的氨基酸。 |
