| 货号 | 14809S |
| 供应商 | CST |
| 背景 | Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA (1,2). Six methyl-lysine-interacting protein families are distinguished based on binding domains: MBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state (3). KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues (4). |
| 存放说明 | -20C |
| 参考文献 | Lanouette, S. et al. (2014) Mol Syst Biol 10, 724. Clarke, S.G. (2013) Trends Biochem Sci 38, 243-52. Herold, J.M. et al. (2011) Curr Chem Genomics 5, 51-61. Thinnes, C.C. et al. (2014) Biochim Biophys Acta 1839, 1416-32. Klose, R.J. et al. (2006) Nature 442, 312-6. Yost, J.M. et al. (2011) Curr Chem Genomics 5, 72-84. |
The Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 130 nonredundant tryptic peptides derived from mouse liver tissue immunoprecipitated with PTMScan® Pan-Methyl Lysine Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position surrounding the central methylated lysine residue within this data set. | |
The chart shows the relative category distribution of proteins with methylated lysine residues derived from peptides identified from a MethylScan® LC-MS/MS experiment of mouse liver tissue using PTMScan® Pan-Methyl Lysine Immunoaffinity Beads. Within this set of 130 peptides, the frequency of mono-methylated lysine peptides is 64%, di-methylated lysine peptide frequency is 24%, and tri-methyl lysine peptides comprise 12% of the sample. |
