| 货号 | 12235S |
| 供应商 | CST |
| 背景 | Arginine methylation is a prevalent PTM found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,N’G-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-dimethylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6).精氨酸甲基化是常见的翻译后修饰，在核和细胞质蛋白中均有发现。精氨酸甲基化蛋白涉及许多细胞过程，包括转录后调节、信号转导、RNA代谢和DNA损伤修复（1-3）。精氨酸甲基化由精氨酸N甲基转移酶（PRMT）家族催化甲基从S-腺苷甲硫氨酸（AdoMet）转移到精氨酸胍氮上而形成（4）。精氨酸甲基化有三种形式：不对称的二甲基化（aDMA, omega-NG,NG-二甲基精氨酸），两个甲基基团在精氨酸胍基氮原子的一端；对称的二甲基化（sDMA, omega-NG, NG-二甲基精氨酸），每个甲基基团在精氨酸胍基氮原子的一端；单甲基化（MMA, omega-NG-二甲基精氨酸），只有一个甲基集团在精氨酸胍基氮原子上。每种修饰都有其潜在的不同功能。尽管所有PRMT蛋白都催化MMA的形成，I型PRMT(PRMT1, 3, 4, 6)可以添加一个额外的甲基基团从而产生aDMA，II型PRMT(PRMT5和7)产生sDMA。甲基化精氨酸残基经常在富含甘氨酸-精氨酸（GAR）蛋白结构域中出现，如RGG, RG, RXR重复序列（5）。然而，PRMT4/CARM1和PRMT5可以甲基化含脯氨酸-甘氨酸-蛋氨酸（PGM）基序的精氨酸序列（6）。 |
| 存放说明 | -20C |
| 参考文献 | Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72. Pahlich, S. et al. (2006) Biochim Biophys Acta 1764, 1890-903. Bedford, M.T. and Clarke, S.G. (2009) Mol Cell 33, 1-13. McBride, A.E. and Silver, P.A. (2001) Cell 106, 5-8. Gary, J.D. and Clarke, S. (1998) Prog Nucleic Acid Res Mol Biol 61, 65-131. Cheng, D. et al. (2007) Mol Cell 25, 71-83. |
The Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 722 nonredundant tryptic peptides derived from human HCT 116 cells immunoprecipitated with PTMScan® Mono-Methyl Arginine motif [mme-RG] Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position surrounding the central methylated arginine residue. Glycine residues are enriched, especially at the +1 position, in the context of mono-methylated arginine when compared to the overall expected frequency in the human proteome. Of the total methylated arginine peptides, 68% contain the [mme-RG] motif. Motif Logo出自MethylScan® LC-MS/MS实验，实验中使用722种低丰度胰蛋白酶消化的多肽，这些来源于人HCT 116细胞的多肽，是使用PTMScan® Mono-Methyl Arginine motif [mme-RG] Immunoaffinity Beads免疫沉淀得到的。该Logo代表了围绕中心的甲基化精氨酸残基的每个位置上氨基酸的相对频率。当与人蛋白质组整个预期的频率比较时，在单甲基化精氨酸的背景下甘氨酸残基被富集，尤其是+1位置。在全部精氨酸甲基化的多肽中，68%含有[mme-RG] 基序。 | |
The pie chart shows the relative category distribution of proteins with mono-methylated arginine identified from peptides generated from a MethylScan® LC-MS/MS experiment of HCT 116 cells using PTMScan® Mono-Methyl Arginine (mme-RG) Immunoaffinity Beads. 饼图显示了从鉴定的多肽中含有单甲基化精氨酸的蛋白的相对种类分布，多肽来自MethylScan® LC-MS/MS实验，使用PTMScan® Mono-Methyl Arginine (mme-RG) Immunoaffinity Beads从HCT116 细胞中免疫得到。 |
