| 货号 | 12810S |
| 供应商 | CST |
| 背景 | Apoptosis is a physiological process resulting in a highly regulated, programmed form of cell death that is a normal part of growth and development in multicellular organisms. Aspartic acid-directed cysteine proteases, caspases, are central to the apoptotic mechanism (1). An intrinsic pathway initiates the apoptotic cascade from signals originating within the cell, such as DNA damage, and an extrinsic pathway initiates apoptosis in response to extracellular signals, like FasL. In either case, initiator caspases, such as caspase-8 and 9, begin cleaving downstream substrates that include the effector caspases, such as caspase-3, the most prominent executioner caspase (2,3). These effector caspases amplify the apoptotic cascade to target many critical proteins needed for normal cell function. Apart from its role in developmental biology, the regulation of apoptosis has broad implications for the study of cancer, autoimmune, and infectious diseases among others (4). In the human proteome, there are thousands of known or putative caspase cleavage sites and all are cleaved at an aspartic acid residue, or, in rare cases, a glutamic acid residue. The resultant fragments containing a carboxy-terminal aspartate generally have the XEXD motif with some variation (5). The PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit provides a unique and sensitive method for quantifying hundreds of cleaved caspase substrates, including caspase-3 and 7, NEDD1, HDAC3, MCM4, Notch1, eIF2B, raptor, Bid, Smad6, NFAT90, and PTEN among others. |
| 存放说明 | -20C |
| 参考文献 | 1 . Kaufmann, T. et al. (2012) Cell Death Differ 19, 42-50. 2 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 3 . Alenzi, F.Q. et al. (2010) Asian Pac J Cancer Prev 11, 271-80. 4 . Favaloro, B. et al. (2012) Aging (Albany NY) 4, 330-49. 5 . Fischer, U. et al. (2003) Cell Death Differ 10, 76-100. |
The Motif Logo was generated from a PTMScan® LC-MS/MS experiment using 1044 nonredundant tryptic peptides with carboxy-terminal aspartates derived from human HeLa cells treated with Staurosporine #9953 1 μM for 3 hours to induce apoptosis. Peptides were immunoprecipitated with PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position leading up to the carboxy-terminal aspartate. Motif Logo出自PhosphoScan® LC-MS/MS实验，实验中使用1044个低丰度胰蛋白酶消化的带有羧基末端天冬氨酸的多肽，这些多肽来源于经1 μM Staurosporine #9953处理3小时后引发凋亡的人HeLa细胞。多肽是用PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Immunoaffinity Beads免疫沉淀得到。该Logo代表了在羧基末端天冬氨酸之前的每个位置上的氨基酸的相对频率。 | |
This chart shows the underlying motif distribution within 1044 nonredundant tryptic peptides with a carboxy-terminal aspartate derived from an LC-MS/MS experiment using HeLa cells treated with Staurosporine #9953 1μM, (3 hr; +) and immunoprecipitated with PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Immunoaffinity Beads. Within the same data set, the proportion of peptides with D in the -3 position is 36%, and the proportion of peptides with E in the -2 position is 53%. 图片显示了PhosphoScan® LC-MS/MS实验中得到的motif分布，实验中使用1044个低丰度胰蛋白酶消化的带有羧基末端天冬氨酸的多肽，这些多肽来源于经1 μM Staurosporine #9953处理3小时后引发凋亡的人HeLa细胞。免疫沉淀中使用的是PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Immunoaffinity Beads。在相同的数据组中，-3位置是D的多肽比例是36%,-2位置是E的多肽比例是53%。 |
