| 货号 | 5564S |
| 供应商 | CST |
| 背景 | AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (2). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (2,3). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (2). AMPK Substrate Motif (LXRXXS */T*) Rabbit mAb contained in this kit, which binds to the AMPK phosphorylation motif LXRXXS*/T* (4), was developed by CST for use in the study and discovery of new AMPK substrates. In this assay, PTMScan® (LXRXXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the (LXRXXS*/T*) motif (S* = phospho-serine, T* = phospho-threonine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Hardie, D.G. (2004) J Cell Sci 117, 5479-87. 2 . Carling, D. (2004) Trends Biochem Sci 29, 18-24. 3 . Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26. 4 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportion of underlying sequence motifs found in a PTMScan® study using AMPK substrate antibody and Orbitrap LC-MS analysis. Analysis of peptides from H1650 cells gave 187 non-redundant sites containing AMPK substrate and related motifs. The primary motifs are highlighted in white. 83% of these sites contain phosphoserine, and the proportion with leucine or methionine residues at the -5 position and arginine at the -3 position (AMPK substrate motif) was 41%. Although this antibody has a preference for the AMPK substrate motif, it also recognizes peptides with just an arginine residue at the -3 position. 图片显示使用AMPK底物抗体和Orbitrap LC-MS分析进行的PTMScan研究中发现的序列模体的属性. 对H1650细胞肽段的实验分析给出包含AMPK底物和相关模体的187个非冗余位点。主要模体用白色加亮显示。包含磷酸化丝氨酸的比率为83%，在-5位有亮氨酸和蛋氨酸和-3位有精氨酸残基（AMPK底物模体）的比率为41%。尽管此抗体对AMPK底物模体有偏好，它也能识别仅在-3位有精氨酸残基的肽段。 | |
The Motif Logo shows the amino acid distributions around the sites recognized by the antibody. 模体标志图显示出分布在此抗体识别位点周围的氨基酸。 | |
AMPK substrate motif western blot analysis of extracts from H1650 cells, untreated or Phenformin treated using AMPK Substrate Motif (LXRXXS */T*) Rabbit mAb. 使用AMPK Substrate Motif (LXRXXS */T*) Rabbit mAb对来自未处理或Phenformin处理的H1650细胞抽提液进行Akt底物模体的Western Blot分析。 |
