PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit【科瑞奥博】

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PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit

货号： 12267S 基本售价：询价规格： 1Kit

产品信息

概述
货号	12267S

性能
供应商	CST
背景	PhosphoScan^® Technology employs a phospho-residue (Tyr, Ser, Thr) motif antibody for phospho-peptide immunoaffinity purification from cell extracts combined with LC tandem MS/MS to identify and quantify changes in phosphorylation levels (1). Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (2). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (2,3). The consensus sequence for ATM/ATR substrates is [pS/pTQ]. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the [pS/pTQ] are negative determinants for substrate phosphorylation (4). The complex phenotype of AT cells suggests that it likely has additional substrates (4). To better understand the kinase and identify substrates for ATM and the related kinase ATR, we have developed antibodies that recognize phosphorylated serine or threonine in the [pS/pTQ] motif.
存放说明	-20C
参考文献	1 . Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86. 2 . Zhao, H. and Piwnica-Worms, H. (2001) Mol Cell Biol 21, 4129-39. 3 . Kim, S.T. et al. (1999) J Biol Chem 274, 37538-43. 4 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

参考图片

This Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 1111 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 and pervanadate and immunoprecipitated using PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Immunoaffinity Beads. The PhosphoSitePlus® logo reflects the relative prevalence of an amino acid in each position relative to the phospho-serine background in the human proteome. Residues represented above the x-axis are enriched relative to their expected frequency in this background. For more information on motif analysis using PSP, please visit www.phosphosite.org.

Motif Logo出自PhosphoScan® LC-MS/MS实验，实验中使用来源于经Calyculin A #9902 和 pervanadate处理的Jurkat细胞的1111种低丰度胰蛋白酶消化的多肽，这些多肽使用PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Immunoaffinity Beads免疫沉淀得到。该PhosphoSitePlus® logo代表了相对于人蛋白质组中的磷酸化丝氨酸的每个位置上氨基酸的相对频率。相对于背景中的预期，那些x轴以上的残基被富集。更多关于使用PSP进行motif 分析的信息，请访问www.phosphosite.org。

This chart shows the uderlying motif distribution within 1111 nonredundant tryptic peptides derived from an LC-MS/MS experiment using Jurkat cells treated with Calyculin A #9902 and pervanadate and immunoprecipitated with PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Immunoaffinity Beads. Within the same data set, phospho-serine in the central position constitutes 88% of the peptides, while phospho-threonine constitutes 12%. The most frequent submotif is [pSQG] with a 16% rate of occurrence relative to the total data set and 28% relative to the subset with the pSQ motif.

图片显示了PhosphoScan® LC-MS/MS实验中得到的motif分布，实验中使用来源于经Calyculin A #9902 和 pervanadate处理的Jurkat细胞的1111种低丰度胰蛋白酶消化的多肽。免疫沉淀中使用的是PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Immunoaffinity Beads。在相同的数据组中，中心位置磷酸化的丝氨酸占88%，而磷酸化的苏氨酸占12%。最频繁的submotif 是[pSQG]，出现的比例相对于整个数据组为16%，相对于含有pSQ motif的亚组为28%。