| 货号 | 5566S |
| 来源宿主 | Rabbit |
| 供应商 | CST |
| 背景 | The ST*P motif is implicated in signaling by polo-like kinases (PLKs). These Ser/Thr protein kinases play essential roles during the cell cycle. At least four PLKs exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4 (2). PLKs have a highly conserved N-terminal kinase domain and a relatively divergent C-terminal domain called the Polo-box domain (PBD). Of the four PLKs, PLK1 is the best characterized (3). PLK1 functions as a key regulator of mitotic events by phosphorylating substrate proteins on centrosomes, kinetochores, the mitotic spindle, and the midbody, and is crucial for proper progression through multiple stages of mitosis (4-6). The PBDs of PLK1 function as a phospho-Ser/Thr-binding module, optimally recognizing the sequence motif S[S*/T*][P/X] (PLK1 binding motif) (6). Binding of phosphopeptides containing S[S*/T*][P/X] motif by the PBD in PLK1 relieves its inhibitory function on kinase activity (7). S[S*/T*]P peptides are phosphorylated by proline-directed kinases such as cyclin dependent kinases (CDKs) (6). These findings imply that priming phosphorylations on substrates or docking proteins by other mitotic kinases such as CDKs may target PLK1 to its substrates and simultaneously activate its kinase domain. The ST*P Motif (ST*P) XP® Rabbit mAb contained in this kit, which binds to ST*P, was developed by Cell Signaling Technology and is a useful tool to study PLK1 binding proteins and PLK1 substrates. In this assay, PTMScan® (ST*P) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the ST*P motif (T* = phospho-threonine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83. 2 . Donaldson, M.M. et al. (2001) J Cell Sci 114, 2357-8. 3 . Barr, F.A. et al. (2004) Nat Rev Mol Cell Biol 5, 429-40. 4 . Kishi, K. et al. (2009) Mol Cell Biol 29, 3134-50. 5 . Elia, A.E. et al. (2003) Science 299, 1228-31. 6 . Elia, A.E. et al. (2003) Cell 115, 83-95. 7 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportion of underlying sequence motifs found in a PTMScan® study using a PLK binding motif antibody and OrbiTrap MS analysis. Analysis of peptides from HeLa cells, untreated and nocodazole-treated, gave 452 non-redundant sites containing a PLK binding and related motifs. The primary motif is highlighted in white 98% of these sites fit the ST*P motif; only 2% of the sites are from other phospho-Ser/Thr containing peptides that do not have the motif, which shows that this antibody is very specific for the ST*P motif. 图片显示使用PLK结合模体抗体和Orbitrap MS分析进行的PTMScan研究中发现的序列模体的属性. 对未处理或nocodazole处理的HeLa细胞肽段的分析给出包含PLK结合模体和相关模体的452个非冗余位点。主要的模体用白色高亮标出。98%的位点符合ST*P模体；只有2%的文电来自其它包含磷酸化丝氨酸/苏氨酸而非此模体的肽段，这表明此抗体对ST*P模体有很高特异性。 | |
The Motif Logo shows the amino acid distributions around the sites recognized by the antibody. 模体标志图显示出分布在此抗体识别位点周围的氨基酸。 | |
Plk bing StP motif western blot analysis of extracts from HeLa cells, untreated or Nocodazole treated, using Phospho-ST*P Motif (ST*P) XP® Rabbit mAb. 使用Phospho-ST*P Motif (ST*P) XP® Rabbit mAb对来自未处理或Nocodazole处理的Hela细胞抽提液进行Plk bing StP模体的Western Blot分析。 |
