| 货号 | 14989S |
| 供应商 | CST |
| 背景 | The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline. |
| 存放说明 | -20C |
| 参考文献 | Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35. Nurse, P. (2000) Cell 100, 71-8. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95. Shah, O.J. et al. (2003) J Biol Chem 278, 16433-42. Songyang, Z. et al. (1994) Curr Biol 4, 973-82. Gonzalez, F.A. et al. (1991) J Biol Chem 266, 22159-63. Yang, X. and Gabuzda, D. (1998) J Biol Chem 273, 29879-87. |
This chart shows the underlying motif distribution in a PhosphoScan® LC-MS/MS experiment using 750 nonredundant tryptic peptides generated from mitotic HeLa cells. HeLa cells were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] Immunoaffinity Beads. | |
The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 750 nonredundant tryptic peptides derived from mitotic HeLa cells. HeLa cells were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] Immunoaffinity Beads. The logo demonstrates the relative frequency of amino acids in a given position around the modified site within this data set. |
