| 货号 | 5565S |
| 供应商 | CST |
| 背景 | An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (2,3). Phospho-PKA substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery. In this assay, PTMScan® (RRXS*/T*) Motif Antibody bead conjugates are used to specifically enrich phospho- peptides containing the RRXS*/T* motif (S* = phospho- serine, T*= phospho-threonine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Montminy, M. (1997) Annu Rev Biochem 66, 807-22. 2 . Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportions of underlying sequence motifs found in a PTMScan study using PKA substrate antibody and Orbitrap LC-MS analysis. Combined analysis of two experiments (#10619, #10620) on peptides from PC-12 cells gave 187 non-redundant sites containing PKA substrate and related motifs. The primary motifs are highlighted in white. The proportion containing phosphoserine was 82%, and the proportion with arginine residues at both the -3 and -2 positions (PKA substrate motif) was 64%. Although this antibody has a strong preference for the PKA substrate motif, it also recognizes peptides with just an arginine residue at the -3 position. 图片显示使用PKA底物抗体和Orbitrap LC-MS分析进行的PTMScan研究中发现的序列模体的属性. 对两个PC-12细胞肽段的实验（#10619、#10620）的结合分析给出包含PKA底物和相关模体的187个非冗余位点。主要模体用白色加亮显示。包含磷酸化丝氨酸的比率为82%，在-3和-2位有精氨酸残基（PKA底物模体）的比率为64%。尽管此抗体对PKA底物模体有偏好，它也能识别仅在-3位有精氨酸残基的肽段。 | |
The Motif Logo shows the amino acid distributions around the sites recognized by the antibody. 模体标志图显示出分布在此抗体识别位点周围的氨基酸。 |
