| 货号 | 4652S |
| 供应商 | CST |
| 背景 | The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (2-4). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXK/R. Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) Rabbit mAb #2325, contained in this kit, was developed at CST for use in the study and discovery of new MAPK and CDK substrates (5,6). In this assay, PTMScan® (PXS*P or S*PXK/R) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the PXS*P motif or the S*PXK/R motif (S* = phospho-serine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41. 2 . Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95. 3 . Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35. 4 . Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93. 5 . Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6. 6 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportions of underlying sequence motifs found in a PTMScan® study using a MAPK/CDK substrate motif antibody and OrbiTrap MS analysis. Analysis of peptides from HeLa cells, untreated (experiment #10684) and nocodazole-treated (experiment #10685), gave 439 nonredundant sites containing MAPK/CDK substrate and related motifs. 20% of these sites are motif PXS*PX(R/K); 70% are motif PXS*P; and 10% are motif S*PX(R/K). The antibody is phospho-serine specific and does not recognize phospho-threonine peptides. 图片显示使用MAPK/CDK底物模体抗体和Orbitrap MS分析进行的PTMScan研究中发现的序列模体的属性. 对未处理（experiment #10684）或nocodazole处理（experiment #10685）的HeLa细胞肽段的分析给出包含MAPK/CDK底物模体和相关模体的439个非冗余位点。这些位点中20%为PXS*PX(R/K)，70%为PXS*P模体，10%为S*PX(R/K)模体。此抗体是磷酸化特异性的，与包含磷酸化苏氨酸的肽段或蛋白没有交叉反应。 |
