| 货号 | 14990S |
| 供应商 | CST |
| 背景 | The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5). |
| 存放说明 | -20C |
| 参考文献 | Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93. |
This chart shows the distribution of underlying phospho-peptide motifs in a PhosphoScan® LC-MS/MS experiment using 754 nonredundant tryptic peptides generated from mitotic HeLa cells. HeLa cells were treated with thymidine, (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Immunoaffinity Beads. | |
The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 754 nonredundant tryptic peptides derived from mitotic HeLa cells that were treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (60 nM, 24 hr) and immunoprecipitated using PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Immunoaffinity Beads. The Motif Logo demonstrates the relative abundance of amino acids in a given position within this data set. |
