| 货号 | 12170S |
| 供应商 | CST |
| 背景 | PhosphoScan® Technology employs a phospho-residue (Tyr, Ser, Thr) motif antibody for phospho-peptide immunoaffinity purification from cell extracts combined with LC tandem MS/MS to identify and quantify changes in phosphorylation levels (1). Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric serine/threonine protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer and other diseases (2-6). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated serine or threonine residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position, followed by the residue at the +1 position (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9. 2 . St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29. 3 . Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67. 4 . Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7. 5 . Perez, D.I. et al. (2011) Med Res Rev 31, 924-54. 6 . Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68. 7 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 471 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 and pervanadate, and immunoprecipitated using PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads. This PhosphoSitePlus® logo represents the enrichment of an amino acid at a residue position relative to the abundance of that amino acid in the background, in this case, the human proteome. Residues represented at the top of the logo are the most enriched, whereas those below the y-axis occur less frequently in the sample than would be expected in the proteome. The PSP logo reflects the specificity of the antibody for the S*/T*DXE motif. For more information on motif analysis using PSP, please visit www.phosphosite.org. Motif Logo出自PhosphoScan® LC-MS/MS实验，实验中使用来源于Calyculin A #9902 及过钒酸钠处理的Jurkat细胞的471 低丰度胰蛋白酶消化的多肽，用PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads进行免疫沉淀。PhosphoSitePlus®标志代表一个氨基酸在一个残基位置上的相对于背景氨基酸丰度的富集，在该图中背景为人类蛋白质组。在logo上方的残基代表最丰富的，而那些y轴以下的表示比其在蛋白质组中的预期还要更少出现。PSP logo反应了S*/T*DXE motif抗体的特异性。 更多关于使用PSP进行motif 分析的信息，请访问www.phosphosite.org. | |
This chart shows the underlying CK2 substrate-like motifs in a PhosphoScan® LC-MS/MS experiment of tryptic peptides generated from Jurkat cells treated with Calyculin A #9902 and pervanadate, and immunoprecipitated using PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads. Of the 354 motif-containing peptides, 54% contained phospho-serine and 46% contained phospho-threonine. The proportion of peptides with aspartate or glutamate at the +1 position is 83% and at the +3 position is 73%. Peptides with aspartate or glutamate at both the +1 and +3 positions is 63%. 图片显示了从PhosphoScan® LC-MS/MS实验中来源于Calyculin A #9902 及过钒酸钠处理的Jurkat细胞，并被胰蛋白酶消化产生的多肽中的CK2 substrate-like motif。免疫沉淀中使用的是PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads。在这些含有354个motif的多肽中，54%含有磷酸化丝氨酸，46%含有磷酸化Thr。多肽中含有Asp或Glu的比例是+1位置83%，+3位置73%。+1和+3都有包含Asp和Glu的多肽比例是63%。 |
