| 货号 | 5636S |
| 来源宿主 | Mouse |
| 供应商 | CST |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (2). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (3). Antibodies specific for phospho-tyrosine (4,5) have been invaluable reagents in these studies. The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411, developed by Cell Signaling Technology, provides an exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. In this assay, PTMScan® (Y*) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing *Y motif (Y* = phospho-tyrosine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Schlessinger, J. (2000) Cell 103, 211-25 2 . Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65. 3 . Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9 4 . Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85 5 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Motif analysis was done using all tyrosine phosphopeptides generated from studies in the published report by Rikova, K.A., et al. (2007) Cell 131, 1190-1203. The Motif Logo, drawn from 4160 non-redundant sites, shows the amino acid distributions around the sites recognized by Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411. The motif analysis shows that this antibody is a general phospho-tyrosine antibody and does not have amino acid preferences at other positions. 使用Rikova, K.A.等发表的研究中产生的所有酪氨酸磷酸化肽段进行的模体分析。[Rikova, K.A., et al.(2007) Cell 131, 1190-1203]从4160个非冗余的位点画出的模体标志图显示出分布在Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411识别位点周围的氨基酸。模体分析显示此抗体是一个通用酪氨酸抗体，对其它位置的氨基酸没有偏好。 |
