| 货号 | 5567S |
| 来源宿主 | Rabbit |
| 供应商 | CST |
| 背景 | A T*PP phosphorylation motif has been extracted from a large-scale proteomic data set on HeLa nuclei (2). Western blot and proteomic analysis shows that phosphorylation on this motif is sensitive to cell cycle control. Similar motifs are phosphorylated by MAPK and CDK families of serine/threonine protein kinases, which play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (motifs PX(S/T)P and (S/T)PXK/R) (3-5). The T*PP Motif (T*PP) XP® Rabbit mAb contained in this kit, which binds to the motif T*PP, was developed by CST for use in the study and discovery of new kinase substrates. In this assay, a PTMScan® (T*PP) Motif Antibody bead conjugates are used to specifically enrich phosphopeptides containing the T*PP motif (T* = phospho-threonine). |
| 存放说明 | -20C |
| 参考文献 | 1 . Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41. 2 . Schwartz, D. and Gygi, S.P. (2005) Nat Biotechnol 23, 1391-8. 3 . Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95. 4 . Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35. 5 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Chart showing the proportions of underlying sequence motifs found in a PTMScan® study using T*PP motif antibody and OrbiTrap MS analysis. The primary motif is highlighted in white. Analysis of peptides from HeLa cells, untreated and nocodazole-treated, gave 236 non-redundant sites. 88% of these sites fit motif T*PP; 8% sites are from motif T*P, and 4% of the sites are from motif S*P. 图片显示使用T*PP模体抗体和Orbitrap MS分析进行的PTMScan研究中发现的序列模体的属性。主要模体用白色加亮显示。对未处理或nocodazole处理的HeLa细胞肽段的分析给出236个非冗余位点。这些位点中88%为T*PP，8%为T*P模体，4%为S*P模体。 | |
The Motif Logo shows the amino acid distributions around the sites recognized by the antibody. 模体标志图显示出分布在此抗体识别位点周围的氨基酸。 | |
T*PP motif western blot analysis of extracts from HeLa cells, untreated or Nocodazole treated, using T*PP Motif (T*PP) XP® Rabbit mAb. 使用T*PP Motif (T*PP) XP® Rabbit mAb对来自未处理或Nocodazole处理的Hela细胞抽提液进行T*PP模体的Western Blot分析。 |
